Datasheet

COH1 Antibody
CATALOG NUMBER: 6931

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:COH1 antibody can be used for detection of COH1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
SPECIFICITY:At least five alternatively spliced transcript variants have been observed. COH1 detects two isoforms.
IMMUNOGEN:COH1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human COH1.

The immunogen is located within amino acids 90 - 140 of COH1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:COH1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:COH1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:COH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:COH1 Antibody: CHS1, COH1, CHS1, KIAA0532, Vacuolar protein sorting-associated protein 13B, Cohen syndrome protein 1
ACCESSION NO.:NP_056058
PROTEIN GI NO.:35493725
OFFICIAL SYMBOL:VPS13B
GENE ID:157680

Background

BACKGROUND:COH1 Antibody: COH1 (Cohen syndrome protein 1), also known as VPS13B (vacuolar protein sorting-associated protein 13B) or CHS1, belongs to the VPS13 family and may function in vesicle-mediated transport and sorting of proteins within the cell. COH1 is widely expressed and multiple alternatively spliced transcript variants have been observed. Mutations in this gene have been associated with Cohen syndrome. COH1 is a Golgi-localized peripheral membrane protein and plays a critical role in Golgi (re)assembly.
REFERENCES: 1) Velayos-Baeza A, Vettori A, Copley RR, et al. Analysis of the human VPS13 gene family. Genomics 2004; 84:536-49.
2) Kolehmainen J, Black GC, Saarinen A, et al. Cohen syndrome is caused by mutations in a novel gene, COH1, encoding a transmembrane protein with a presumed role in vesicle-mediated sorting and intracellular protein transport. Am. J. Hum. Genet. 2003; 72:1359-69.
3) Seifert W, K├╝hnisch J, Maritzen T, et al. Cohen syndrome-associated protein, COH1, is a novel, giant Golgi matrix protein required for Golgi integrity. J. Biol. Chem. 2011; 286:37665-75.

For Research Use Only