CNTF Antibody (biotin) Cat. No.: XP-5114Bt

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Rat
IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat CNTF (rat CNTF).
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ELISA:

Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat CNTF (XP-5114) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.

Western Blot:

To detect rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat CNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

psi-iconProperties
PURIFICATION:Anti-rat CNTF specific antibody was purified by affinity chromatography and then biotinylated.
CLONALITY:Polyclonal
CONJUGATE:Biotin
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:CNTF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:Cntf
ALTERNATE NAMES:Ciliary neurotrophic factorCNTF
ACCESSION NO.:P20294
PROTEIN GI NO.:116587
GENE ID:25707
USER NOTE:Centrifuge vial prior to opening.
psi-iconBackground and References
BACKGROUND:Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.

ANTIBODIES FOR RESEARCH USE ONLY.

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