CNTF Antibody Cat. No.: XP-5114

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Rat
IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rCNTF (Rat Ciliary Neurotrophic Factor).
TESTED APPLICATIONS: ELISA, Neut, WB
APPLICATIONS: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/mL), a concentration of 0.02 - 0.05 μg/mL of this antibody is required.

ELISA:

To detect rCNTF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF.

Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.

Western Blot:
To detect rCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

psi-iconProperties
PURIFICATION:Anti-rCNTF specific antibody was purified by affinity chromatography employing immobilized rCNTF matrix.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:CNTF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:Cntf
ALTERNATE NAMES:Ciliary neurotrophic factorCNTF
ACCESSION NO.:P20294
PROTEIN GI NO.:116587
GENE ID:25707
USER NOTE:Centrifuge vial prior to opening.
psi-iconBackground and References
BACKGROUND:Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.

ANTIBODIES FOR RESEARCH USE ONLY.

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