CNOT4 Antibody Cat. No.: 4813

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: CNOT4 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CNOT4.

The immunogen is located within amino acids 70 - 120 of CNOT4.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: CNOT4 antibody can be used for detection of CNOT4 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate

psi-iconProperties
PURIFICATION:CNOT4 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CNOT4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CNOT4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CNOT4
ALTERNATE NAMES:CNOT4 Antibody: NOT4, NOT4H, CLONE243, NOT4, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4
ACCESSION NO.:NP_037448
PROTEIN GI NO.:56550057
GENE ID:4850
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CNOT4 Antibody: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.
REFERENCES:1) Albert TK, Hanzawa H, Legtenberg IA, et al. Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J.2002; 21:355-64.
2) Winkler GS, Albert TK, Dominguez C, et al. An altered specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair. J. Mol. Biol.2004; 337:157-65.
3) Mulder KW, Inagaki A, Cameroni E, et al. Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae. Genetics2007; 176:181-92.

ANTIBODIES FOR RESEARCH USE ONLY.

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