Datasheet

CLPH Antibody
CATALOG NUMBER: 5497

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (75%), Mouse: (70%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:CLPH antibody can be used for detection of CLPH by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
IMMUNOGEN:CLPH antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human CLPH.

The immunogen is located within the last 50 amino acids of CLPH.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CLPH Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CLPH Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CLPH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CLPH Antibody: CLPH, C4orf35, NYD-SP26, Calcium-binding and spermatid-specific protein 1, Testis development protein NYD-SP26
ACCESSION NO.:NP_149113
PROTEIN GI NO.:90652863
OFFICIAL SYMBOL:CABS1
GENE ID:85438

Background

BACKGROUND:CLPH Antibody: CLPH (Casein-Like phosphoprotein) is a recently discovered, evolutionarily conserved, testis-specific cytoplasmic protein expressed exclusively in postmeiotic germ cells in rat spermatogenesis. CLPH is a novel casein kinase 2-phosphorylated disordered and calcium-binding protein that displays strong variation in relative expression levels during spermatogenesis. It is frequently associated with the mitochondrial inner membrane during the last steps of spermatid differentiation and is involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis.
REFERENCES: 1) Calvel P, Kervarrec C, Lavigne R, et al. CLPH, a novel casein kinase 2-phosphorylated disordered protein, is specifically associated with postmeiotic germ cells in rat spermatogenesis. J. Proteome Res.2009; 8:2953-65.

For Research Use Only