CLIP170 Antibody Cat. No.: 4659

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: CLIP170 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human CLIP170.

The immunogen is located within amino acids 1340 - 1390 of CLIP170.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: CLIP170 antibody can be used for detection of CLIP170 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide

psi-iconProperties
PURIFICATION:CLIP170 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CLIP170 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CLIP170 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CLIP1
ALTERNATE NAMES:CLIP170 Antibody: RSN, CLIP, CYLN1, CLIP170, CLIP-170, RSN, CAP-Gly domain-containing linker protein 1, Cytoplasmic linker protein 1
ACCESSION NO.:NP_002947
PROTEIN GI NO.:4506751
GENE ID:6249
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CLIP170 Antibody: CLIP170 was initially identified as a new type of intermediate filament associated protein that is highly expressed in Reed-Sternberg cells, the tumoral cells diagnostic for Hodgkin's disease. Later experiments showed that it is located at microtubule plus ends and is required for the binding of endocytic carrier vesicles. CLIP170 has also been suggested to act with LIS1, a protein implicated in brain development, to regulate dynein/dynactin binding microtubules. Other studies suggest that CLIP170 can influence the formation of lamellipodia and cell invasion by invasive breast cancer cells by regulating the release of kinesin and IQGAP1 from a complex of those proteins, CLIP170 and Rac1. At least two isoforms of CLIP170 are known to exist.
REFERENCES:1) Bilbe G, Delabie J, Bruggen J, et al. Restin: a novel intermediate filament-associated protein highly expressed in the Reed-Sternberg cells of Hodgkin’s disease. EMBO J.1992; 11:2103-13.
2) Diamantopoulos GS, Perez F, Goodson HV, et al. Dynamic localization of CLIP-170 to microtubule plus ends is coupled to microtubule assembly. J. Cell Biol.1999; 144:99-112.
3) Pierre P, Scheel J, Richard JE, et al. CLIP-170 links endocytic vesicles to microtubules. Cell70:887-900.
4) Suzuki K and Takahashi K. Regulation of lamellipodia formation and cell invasion by CLIP-170 in invasive human breast cancer cells. Biochem. Biophys. Res. Commun.2008; 368:199-204.

ANTIBODIES FOR RESEARCH USE ONLY.

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