Datasheet

CLEC7A Antibody
CATALOG NUMBER: 6833

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (88%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CLEC7A antibody can be used for detection of CLEC7A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1466 - Rat Spleen Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 27 kDa

Observed: 27 kDa
SPECIFICITY:Multiple isoforms of CLEC7A are known to exist.
IMMUNOGEN:CLEC7A antibody was raised against a 17 amino acid synthetic peptide near the center of human CLEC7A.

The immunogen is located within amino acids 70 - 120 of CLEC7A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CLEC7A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CLEC7A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CLEC7A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CLEC7A Antibody: BGR, CANDF4, DECTIN1, CLECSF12, BGR, UNQ539/PRO1082, C-type lectin domain family 7 member A, Beta-glucan receptor, DC-associated C-type lectin 1
ACCESSION NO.:NP_922938
PROTEIN GI NO.:37675373
OFFICIAL SYMBOL:CLEC7A
GENE ID:64581

Background

BACKGROUND:CLEC7A Antibody: CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
REFERENCES: 1) Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J. Immunol. 2002; 169:3876-82.
2) Ebner S, Sharon S, and Ben-Tal N. Evolutionary analysis reveals collective properties and specificity in the C-type lectin and lectin-like domain superfamily. Proteins 2003; 53:44-55.
3) Kerrigan AM and Brown GD. Syk-coupled C-type lectin receptors that mediate cellular activation via single tyrosine based activation motifs. Immunol. Rev. 2010; 234:335-52.
4) Underhill DM, Rossnagle E, Lowell CA, et al. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood 2005; 106:2543-50.

For Research Use Only