Datasheet

CLEC2A Antibody
CATALOG NUMBER: 6959

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:CLEC2A antibody can be used for detection of CLEC2A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide
SPECIFICITY:CLEC2A antibody is human specific. Two alternatively spliced transcript variants have been observed.
IMMUNOGEN:CLEC2A antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CLEC2A.

The immunogen is located within the first 50 amino acids of CLEC2A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CLEC2A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CLEC2A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CLEC2A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CLEC2A Antibody: KACL, PILAR, UNQ5792, KACL, UNQ5792/PRO19597, C-type lectin domain family 2 member A, Keratinocyte-associated C-type lectin
ACCESSION NO.:NP_001124183
PROTEIN GI NO.:195222725
OFFICIAL SYMBOL:CLEC2A
GENE ID:387836

Background

BACKGROUND:CLEC2A Antibody: CLEC2A (C-type lectin domain family 2 member A), also known as PILAR (proliferation-induced lymphocyte-associated receptor) is involved in modulating T-cell expansion. It is a 32 kDa single-pass type II membrane protein that contains one C-type lectin domain in its extracellular region and belongs to the CTL/CTLD superfamily. CLEC2A is mainly expressed in skin and highly expressed in CD8(+), B lymphocytes and naive CD4(+) T cells. Manipulation of CLEC2A signaling may be important for treatment of autoimmune diseases and cancer.
REFERENCES: 1) Huarte E, Cubillos-Ruiz JR, Nesbeth YC, et al. PILAR is a novel modulator of human T-cell expansion. Blood 2008; 112:1259-68.
2) Drickamer K. C-type lectin-like domains. Curr. Opin. Struct. Biol. 1999; 9:585-90
3) Spreu J, Kienle EC, Schrage B, et al. CLEC2A: a novel, alternatively spliced and skin-associated member of the NKC-encoded AICL-CD69-LLT1 family. Immunogenetics 2007; 59:903-12.
4) Spreu J, Kuttruff S, Stejfova V, et al. Interaction of C-type lectin-like receptors NKp65 and KACL facilitates dedicated immune recognition of human keratinocytes. Proc. Natl. Acad. Sci. USA 2010; 107:5100-5.

For Research Use Only