Datasheet

CISD2 Antibody
CATALOG NUMBER: 7301

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:IF, IHC-P, WB
APPLICATIONS:CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 15 kDa

Observed: 15 kDa
IMMUNOGEN:CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2.

The immunogen is located within amino acids 40 - 90 of CISD2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CISD2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CISD2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CISD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CISD2 Antibody: ERIS, WFS2, ZCD2, NAF-1, Miner1, CDGSH2, ERIS, CDGSH iron-sulfur domain-containing protein 2, Endoplasmic reticulum intermembrane small protein
ACCESSION NO.:NP_001008389
PROTEIN GI NO.:56605994
OFFICIAL SYMBOL:CISD2
GENE ID:493856

Background

BACKGROUND:CISD2 Antibody: Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. CISD2 interacts with Bcl-2 and can be displaced by the BH3-only protein BIK and contributes to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death as well as the development of premature aging phenotype.
REFERENCES: 1) Amr S, Heisey C, Zhang M, et al. A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2. Am. J. Hum. Genet. 2007; 81:673-83.
2) Chang NC, Nguyen M, Germain M, et al. Antagonism of Beclin 1-dependent autophagy by BCL-2 at the endoplasmic reticulum requires NAF-1. EMBO J. 2010; 29:606-18.
3) Chen YF, Kao CH, Chen YT, et al. Cisd2 deficiency drives premature aging and causes mitochondria-mediated defects in mice.

For Research Use Only