Datasheet

CIP75 Antibody
CATALOG NUMBER: 5267

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
IMMUNOGEN:CIP75 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CIP75.

The immunogen is located within the first 50 amino acids of CIP75.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CIP75 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CIP75 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CIP75 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CIP75 Antibody: A1U, A1Up, UBIN, CIP75, C1orf6, Ubiquilin-4, Ataxin-1 interacting ubiquitin-like protein
ACCESSION NO.:Q9NRR5
PROTEIN GI NO.:45476982
OFFICIAL SYMBOL:UBQLN4
GENE ID:56893

Background

BACKGROUND:CIP75 Antibody: The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.
REFERENCES: 1) Su V and Lau AF. Ubiquitin-like and ubiquitin-associated domain proteins: significance in proteasomal degradation. Cell. Mol. Life Sci.2009; 66:2819-33.
2) Davidson JD, Riley B, Burright EN, et al. Identification and characterization of an ataxin-1-interacting protein: A1Up, a ubiquitin-like protein. Hum. Mol. Genet.2000; 9:2305-12.
3) Li X, Su V, Kurata WE, et al. A novel Connexin43-interacting protein, CIP75, which belongs to the UbL-UBA protein family, regulates the turnover of Connexin4 J. Biol. Chem.2008; 283:5748-59.

For Research Use Only