CIDE-A Antibody Cat. No.: 2089

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: CIDE-A antibody was raised against an 18 amino acid peptide near the carboxy terminus of mouse CIDE-A.

The immunogen is located within the last 50 amino acids of CIDE-A.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: CIDE-A antibody can be used for detection of CIDE-A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
SPECIFICITY: It has no cross activity to CIDE-B.
POSITIVE CONTROL:1) Cat. No. 1401 - Mouse Heart Tissue Lysate
PREDICTED MOLECULAR WEIGHT: 25 kDa

psi-iconProperties
PURIFICATION:CIDE-A Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CIDE-A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CIDE-A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CIDEA
ALTERNATE NAMES:CIDE-A Antibody: CIDE-A, Cell death activator CIDE-A, Cell death-inducing DFFA-like effector A
ACCESSION NO.:NP_031728
PROTEIN GI NO.:3114595
GENE ID:1149
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CIDE-A Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.
REFERENCES:1) Inohara N, Koseki T, Chen S, et al. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
2) Inohara N, Koseki T, Chen S, et al. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4 (RD1299)

ANTIBODIES FOR RESEARCH USE ONLY.

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