Datasheet

cIAP Antibody
CATALOG NUMBER: 3325

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (93%), Chicken: (92%), Rat: (91%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:cIAP antibody can be used for the detection of c-IAP by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1331 - Human Lung Tissue Lysate
IMMUNOGEN:c-IAP antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human c-IAP1 c-IAP antibody detects both c-IAP1 and c-IAP2.

The immunogen is located within amino acids 540 - 590 of cIAP.
HOST SPECIES:Rabbit

Properties

PURIFICATION:cIAP Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:cIAP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:cIAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1, API1, IAP2, Baculoviral IAP repeat-containing protein 2, C-IAP1, IAP-2
ACCESSION NO.:NP_001157
PROTEIN GI NO.:4502141
OFFICIAL SYMBOL:BIRC2
GENE ID:329

Background

BACKGROUND:cIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.
REFERENCES: 1) Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90.
2) Rothe M, Pan M-G, Henzel WJ, et al. The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins. Cell 1995; 83:1243-52.
3) Deveraux QL, Leo E, Stennicke HR, et al. IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J. 1998; 17:2215-23.
4) Li X, Yang Y, Ashwell JD. TNF-RII and c-IAP1 mediate ubiquitinization and degradation of TRAF 2. Nature 2002; 416:345-7.

For Research Use Only