cIAP Antibody Cat. No.: 3325

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (93%), Chicken: (92%)
IMMUNOGEN: Anti-cIAP antibody (3325) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP. c-IAP antibody detects both c-IAP1 and c-IAP2.

The immunogen is located within amino acids 540-590 of cIAP.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1-4 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1331 - Human Lung Tissue Lysate
 2) Cat. No. 1203 - A549 Cell Lysate
 3) Cat. No. 1220 - SK-N-SH Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 70kD

Observed: 70kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar cIAP expression profile in human, mouse and rat cell lines detected by two independent anti-cIAP antibodies that recognize different epitopes, 3325 against C-terminus domain and competitor antibody against recombinant protein.  cIAP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

ISOFORMS: Human cIAP has 2 isoforms, including isoform 1 (618aa, 70kD) and isoform 2 (569aa, 64kD). Mouse cIAP has one isoform (612aa, 70kD) and Rat cIAP also has one isoform (589aa, 67kD). 3325 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:cIAP Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:cIAP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:cIAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BIRC2
ALTERNATE NAMES:cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1, API1, IAP2, Baculoviral IAP repeat-containing protein 2, C-IAP1, IAP-2
ACCESSION NO.:NP_001157
PROTEIN GI NO.:4502141
GENE ID:329
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:cIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.
REFERENCES:1) Schimmer. Cancer Res. 2004; 64:7183-90.
2) Rothe et al. Cell 1995; 83:1243-52.
3) Deveraux et al. EMBO J. 1998; 17:2215-23.
4) Li et al. Nature 2002; 416:345-7.
psi-iconCITATIONS
CITATIONS: 1)Nuutinen et al. The effect of microenvironmental CD40 signals on TRAIL- and drug-induced apoptosis in follicular lymphoma cells. Scand J Immunol. 2009;70(6):565-73.PMID: 19906199
2)Hahm and Singh. Withaferin A-induced apoptosis in human breast cancer cells is associated with suppression of inhibitor of apoptosis family protein expression. Cancer Lett. 2013;334(1):101-8. PMID: 22935676

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