Datasheet

CHORDC1 Antibody
CATALOG NUMBER: 5587

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%), Chicken: (86%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:CHORDC1 antibody can be used for detection of CHORDC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
IMMUNOGEN:CHORDC1 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of human CHORDC1.

The immunogen is located within amino acids 50 - 100 of CHORDC1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CHORDC1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CHORDC1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CHORDC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CHORDC1 Antibody: CHP1, CHP1, Cysteine and histidine-rich domain-containing protein 1, CHORD domain-containing protein 1, CHORD-containing protein 1
ACCESSION NO.:NP_036256
PROTEIN GI NO.:221316566
OFFICIAL SYMBOL:CHORDC1
GENE ID:26973

Background

BACKGROUND:CHORDC1 Antibody: The cysteine and histidine-rich domain (CHORD)-containing protein (CHORDC1) is a member of a highly conserved protein family that contains the plant protein RAR1 and the mammalian protein melusin. In mammals, CHORDC1 is an ADP-dependent HSP90-interacting protein, and this interaction is dependent on the ability of HSP90 to bind nucleotides. Recent experiments indicate that CHORDC1 mRNA is diurnally regulated in mouse hypothalamus, and that this regulation alters during development, suggesting that CHORDC1 may play a role in circadian mechanisms in the mammalian brain.
REFERENCES: 1) Shirasu K, Lahaye T, Tan MW, et al. A novel class of eukaryotic zinc-binding proteins is required for disease resistance signaling in barley and development in C. elegans. Cell1999; 99:355-66.
2) Brancaccio M, Menini N, Bongianni D, et al. Chp-1 and melusin, two CHORD containing proteins in vertebrates. FEBS Lett.2003; 551:47-52.
3) Wu J, Luo S, Jiang H, et al. Mammalian CHORD-containing protein 1 is a novel heat shock protein 90-interacting protein. FEBS Lett.2005; 579:421-6.
4) Gano JJ and Simon JA. A proteomic investigation of ligand-dependent HSP90 complexes reveals CHORDC1 as a novel ADP-dependent HSP90-interacting protein. Mol. Cell Proteomics2010; 9:255-70.

For Research Use Only