Datasheet

Chondroadherin Antibody
CATALOG NUMBER: 8421

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:Chondroadherin antibody can be used for detection of Chondroadherin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 38 kDa

Observed: 36 kDa
SPECIFICITY:Chondroadherin antibody is human and mouse reactive. Chondroadherin antibody is predicted to not cross-react with CHADL protein.
IMMUNOGEN:Chondroadherin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Chondroadherin.

The immunogen is located within the last 50 amino acids of Chondroadherin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Chondroadherin antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Chondroadherin antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Chondroadherin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Cartilage leucine-rich protein, CHAD, SLRR4A
ACCESSION NO.:NP_001258
PROTEIN GI NO.:153251229
OFFICIAL SYMBOL:CHAD
GENE ID:1101

Background

BACKGROUND:Chondroadherin, also known as CHAD or SLRR4A, belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). It localizes to the extracellular matrix and contains nine tandem leucine repeats as well as N-terminal and C-terminal leucine rich domains (2-4). Chondroadherin is implicated in the adhesion of fibroblasts, osteoblasts and chondrocytes, which is mediated by interactions with Integrin alpha2 beta1 (2,4,5).
REFERENCES: 1) Neame PJ, Sommarin Y, Boynton RE, et al. The structure of a 38-kDa leucine-rich protein (chondroadherin) isolated from bovine cartilage. J. Biol. Chem. 1994; 269:21547-54.
2) Haglund L, Tillgren V, Addis L, et al. Identification and characterization of the integrin alpha2beta1 binding motif in chondroadherin mediating cell attachment. J. Biol. Chem. 2011; 286:3925-34.
3) Mansson B, Wenglen C, Morgelin M, et al. Association of chondroadherin with collagen type II. J. Biol. Chem. 2001; 276:32883-8.
4) Camper L, Heinegard D, and Lundgren-Akerlund E. Integrin alpha2beta1 is a receptor for the cartilage matrix protein chondroadherin. J. Cell Biol. 1997; 138:1159-67.

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