Datasheet

CHD7 Antibody
CATALOG NUMBER: 6441

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CHD7 antibody can be used for detection of CHD7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
SPECIFICITY:Multiple isoforms of CHD7 are known to exist.
IMMUNOGEN:CHD7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CHD7.

The immunogen is located within amino acids 670 - 720 of CHD7.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CHD7 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CHD7 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CHD7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CHD7 Antibody: CRG, HH5, IS3, KAL5, KIAA1416, Chromodomain-helicase-DNA-binding protein 7, ATP-dependent helicase CHD7, CHD-7
ACCESSION NO.:Q9P2D1
PROTEIN GI NO.:54112403
OFFICIAL SYMBOL:CHD7
GENE ID:55636

Background

BACKGROUND:CHD7 Antibody: CHD proteins belong to a superfamily of proteins of ATP-dependent chromatin remodeling enzymes that have a unique combination of functional domains, including two N-terminal chromodomains, a SNF2-like ATPase/helicase domain and a DNA-binding domain. These proteins are thought to play a role in early embryonic development by affecting chromatin structure and gene expression. Mutations in one member of this family, CHD7, result in CHARGE syndrome. It colocalizes with embryonic stem (ES) cell master regulators OCT4/POU5F1, SOX2, and NANOG and is thought to modulate ES-specific gene transcription. Together with SOX2, CHD7 has been suggested to also regulate several human disease genes.
REFERENCES: 1) Woodage T, Basrai MA, Baxevanis AD, et al. Characterization of the CHD family of proteins. Proc. Natl. Acad. Sci. USA 1997; 94:11472-7.
2) Cavalli G and Paro R. Chromo-domain proteins: linking chromatin structure to epigenetic regulation. Curr. Opin. Cell Biol. 1998; 10:354-60.
3) Vissers LE, van Ravenswaaj CM, Admiraal R, et al. Mutations in a new member of the chromodomain gene family cause CHARGE syndrome. Nat. Genet. 2004; 36:955-7.
4) Schnetz MP, Handoko L, Akhtar-Zaidi B, et al. CHD7 targets active gene enhancer elements to modulate ES cell-specific gene expression. PLoS Genet. 2010; 6:31001023.

For Research Use Only