CHCHD6 Antibody Cat. No.: 7133

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Rabbit polyclonal CHCHD6 antibody was raised against a 17 amino acid peptide near the amino terminus of human CHCHD6.

The immunogen is located within amino acids 20 - 70 of CHCHD6.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: CHCHD6 antibody can be used for detection of CHCHD6 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: CHCHD6 is predicted to not cross-react to other members of the CHCHD family.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 26 kDa

psi-iconProperties
PURIFICATION:CHCHD6 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CHCHD6 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CHCHD6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:CHCHD6
ALTERNATE NAMES:CHCHD6 Antibody: CHCM1, PPP1R23, CHCM1, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, mitochondrial, Coiled coil helix cristae morphology protein 1
ACCESSION NO.:NP_115719
PROTEIN GI NO.:14150134
GENE ID:84303
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CHCHD6 Antibody: CHCHD6 is a member of a family of proteins containing a conserved (coiled coil 1)-(helix 1)-(coiled coil 2)-(helix 2) domain and has been observed in a complex with the mitochondrial proteins mitofilin, SAM50, metaxins 1 and 2, and CHCHD3. CHCHD6 knockdown causes severe defects in mitochondrial cristae morphology as well as reductions in cell growth, ATP production and oxygen consumption. This decrease of CHCHD6 also led to a reduction in mitofilin protein levels, while a knockdown of mitofilin resulted in a reduced level of CHCHD6, suggesting coordinate regulation between these two proteins. CHCHD6 knockdown in human cancer cells enhances their sensitivity to genotoxic anticancer drugs, suggesting that CHCHD6 may be a potential therapeutic anti-tumor target.
REFERENCES:1) Xie J, Marusich MF, Souda P, et al. The mitochondrial inner membrane protein Mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11. FEBS Lett. 2007; 581:3545-9.
2) An J, Shi J, He Q, et al. CHCM1/CHCHD6, novel mitochondrial protein linked to regulation of mitofilin and mitochondrial cristae morphology. J. Biol. Chem. 2012; 287:7411-26.

ANTIBODIES FOR RESEARCH USE ONLY.

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