ChAT Antibody Cat. No.: 45-037

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Mouse, Rat
IMMUNOGEN: The immunogen for this antibody is: C-KERARGPTKPSNLD
TESTED APPLICATIONS: ELISA, IHC, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.
SPECIFICITY: This antibody is NOT expected to cross-react to Human CHAT.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:Chat
ALTERNATE NAMES:Chat, choline acetyltransferase , B230380D24Rik
ACCESSION NO.:NP_034021.1
PROTEIN GI NO.:45476575
GENE ID:12647 (mouse); 432359 (rat);
psi-iconBackground and References
REFERENCES:1) Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E. Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. J Neurochem. 2006 Apr;97(2):515-26. Epub 2006 Mar 15.

ANTIBODIES FOR RESEARCH USE ONLY.

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