Datasheet

CFTR Antibody
CATALOG NUMBER: 7717

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Sheep: (100%), Rabbit: (100%), Bovine: (95%), Pig: (89%), Horse: (89%), Guinea pig: (89%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CFTR antibody can be used for detection of CFTR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1308 - Human Small Intestine Tissue Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 163 kDa

Observed: 160 kDa
SPECIFICITY:CFTR antibody is human, mouse and rat reactive.
IMMUNOGEN:CFTR antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CFTR.

The immunogen is located within amino acids 1290 - 1340 of CFTR.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CFTR antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CFTR Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CFTR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CFTR Antibody: CF, MRP7, ABC35, ABCC7, CFTR/MRP, TNR-CFTR, dJ760C5.1, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, CFTR
ACCESSION NO.:NP_000483
PROTEIN GI NO.:90421313
OFFICIAL SYMBOL:CFTR
GENE ID:1080

Background

BACKGROUND:The cystic fibrosis transmembrane conductance regulator (CFTR) protein is a member of the ATP-binding cassette (ABC) transporter superfamily, and a member of the MRP subfamily that is involved in multi-drug resistance (1,2). CFTR functions as a chloride channel and controls the regulation of other transport pathways (3). Mutations in this gene are associated with the autosomal recessive disorder cystic fibrosis, the most common, fatal, inherited disease of caucasian populations (1).
REFERENCES: 1) Kerem B, Rommens JM, Buchanan JA, et al. Identification of the cystic fibrosis gene: genetic analysis. Science 1989; 245:1073-80.
2) Harris A and Argent BE. The cystic fibrosis gene and its product CFTR. Semin. Cell Biol. 1993; 4:37-44.
3) Schwiebert EM, Benos DJ, Egan ME, et al. CFTR is a conductance regulator as well as a chloride channel. Physiol. Rev. 1999; 79:S145-66.

For Research Use Only