Datasheet

CENPW Antibody
CATALOG NUMBER: 6943

Availability: In stock

$0.00
Product Price Options

* Required Fields

$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT:kDa
SPECIFICITY:At least three isoforms of CENPW are known to exist; this antibody will detect all three isoforms.
IMMUNOGEN:CENPW antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CENPW.

The immunogen is located within the first 50 amino acids of CENPW.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CENPW Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CENPW Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CENPW antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CENPW Antibody: CUG2, CENP-W, C6orf173, CUG2, Centromere protein W, Cancer-up-regulated gene 2 protein
ACCESSION NO.:NP_001012525
PROTEIN GI NO.:60302883
OFFICIAL SYMBOL:CENPW
GENE ID:387103

Background

BACKGROUND:CENPW Antibody: CENPW was initially identified as a gene that was upregulated in multiple cancers, and whose overexpression in mouse fibroblast cells gave rise to distinct cancer-specific phenotypes. It was later found to be a nuclear protein that associated with CENPT, a component of CENPA nucleosome complex in the centromere, and is required for proper kinetochore function. CENPW also specifically interacts with the nucleolar phosphoprotein nucleophosmin, also known as B23 It has been suggested that nucleophosmin functions in the assembly of the kinetochore by interacting with CENPW during interphase. Overexpression of CENPW in the SKOV-3 human ovarian cancer cell line as well as in the zebrafish embryo led to apoptosis, suggesting that high levels of CENPW induces apoptotic cell death.
REFERENCES: 1) Lee S, Gang J, Jeon SB, et al. molecular cloning and functional analysis of a novel oncogene, cancer-upregulated gene 2 (CUG2). Biochem. Biophys. Res. Commun. 2007; 360:633-9.
2) Kim H, Lee M, Lee S, et al. Cancer-upregulated gene 2 (CUG2), a new component of centromere complex, is required for kinetochore function. Mol. Cells 2009; 27:697-701.
3) Chun Y, Park B, Koh W, et al. New centromeric component CENT-W is an RNA-associated nuclear matrix protein that interacts with nucleophosmin/B23 protein. J. Biol. Chem. 2011; 286:42758-69.
4) Lee S, Koh W, Kim HT, et al. Cancer-upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV-3 cells. Cell Biochem. Funct. 2010; 28:461-8.

For Research Use Only