Datasheet

CEBPD Antibody
CATALOG NUMBER: 7077

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:CEBPD antibody can be used for detection of CEBPD by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1466 - Rat Spleen Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:30 kDa
SPECIFICITY:Multiple transcript variants encoding different isoforms have been found for this gene.
IMMUNOGEN:Rabbit polyclonal CEBPD antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CEBPD.

The immunogen is located within amino acids 170 - 220 of CEBPD.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CEBPD Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CEBPD Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CEBPD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CEBPD Antibody: CELF, CRP3, C/EBP-delta, NF-IL6-betaNuclear factor NF-IL6-beta, NF-IL6-beta
ACCESSION NO.:NP_005186
PROTEIN GI NO.:28872798
OFFICIAL SYMBOL:CEBPD
GENE ID:1052

Background

BACKGROUND:CEBPD Antibody: CCAAT enhancer binding proteins (CEBPs) are a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. At least six members of the family have been isolated and characterized to date (CEBP alpha to CEBP zeta). CEBPD is a leucine zipper (LZ) DNA-binding protein that regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. CEBPD is an important transcriptional activator in the regulation of genes involved in immune and inflammatory responses and has been reported to possess many tumor suppressor-like properties.
REFERENCES: 1) Williams SC, Cantwell CA, Johnson PF, et al. A family of C/EBP-related proteins capable of forming covalently linked leucine zipper dimers in vitro. Genes Dev.1991; 5:1553-67.
2) Umek RM, Friedman AD, and McKnight SL. CCAAT-enhancer binding protein: a component of a differentiation switch. Science 1991; 251:288-92.
3) Ramji DP and Foka P. CCAAT/enhancer-binding proteins: structure, function and regulation. Biochem. J. 2002; 365:561-75.
4) Johnson PF, Landschulz WH, Graves BJ, et al. Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses. Genes Dev. 1987; 1:133-46.

For Research Use Only