Datasheet

CDX2 Antibody
CATALOG NUMBER: 5595

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (94%), Bovine: (90%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CDX2 antibody can be used for detection of CDX2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
IMMUNOGEN:CDX2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CDX2.

The immunogen is located within amino acids 220 - 270 of CDX2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CDX2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CDX2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CDX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CDX2 Antibody: CDX3, CDX-3, CDX3, Homeobox protein CDX-2
ACCESSION NO.:CAA74038
PROTEIN GI NO.:2193875
OFFICIAL SYMBOL:CDX2
GENE ID:1045

Background

BACKGROUND:CDX2 Antibody: CDX2 is a member of the caudal homeobox family of transcription factors that also includes the proteins CDX1 and CDX4. CDX2 is strongly expressed in the trophectoderm in the blastocyst with expression maintained within the proliferating extra-embryonic ectoderm during development. It is required for the repression of the transcription of the POU5F1/Oct4 and NANOG genes in the outer cells of the blastocyst and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Aberrant expression of CDX2 is associated with numerous types of carcinomas, including intestinal metaplasia, gastric carcinoma, and acute myeloid leukemia due to deregulated HOX gene expression.
REFERENCES: 1) Beck F, Erler T, Russell A, et al. Expression of CDX-2 in the mouse embryo and placenta: possible role in patterning of the extra-embryonic membranes. Dev. Dyn.1995; 204:219-27.
2) Strumpf D, Mao CA, Yamanaka Y, et al. Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Dev.2005; 132:2093-102.
3) Ishikawa A, Sasaki M, Ohira S, et al. Aberrant expression of CDX2 is closely related to the intestinal metaplasia and MUC2 expression in intraductal papillary neoplasm of the liver in hepatolithiasis. Lab. Invest.2004; 84:629-38.
4) Silberg DG, Sullivan J, Kang E, et al. Cdx2 ectopic expression induces gastric intestinal metaplasia in transgenic mice. Gastroenterology2002; 122:689-96.

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