CDKN2A Antibody Cat. No.: 4211

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-CDKN2A antibody (4211) was raised against a peptide corresponding to 18 amino acids near the amino terminus of human CDKN2A.

The immunogen is located within the first 50 amino acids of CDKN2A.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1 - 2 μg/mL; IHC-P: 5 - 10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1411 - Mouse Colon Tissue Lysate
 2) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 17 kDa

Observed: 17 kDa
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 2) shows that anti-CDKN2A antibody (4211) detects human recombinant CDKN2A protein that contains the amino acids of 4211 antigenic peptide.

ISOFORMS: Human CDKN2A has 6 isoforms, including isoform 1 (156aa, 17kD), isoform 2 (105aa, 11kD), isoform 3 (116aa, 12kD), isoform 5 (167aa, 18kD). Mouse CDKN2A has 2 isoforms, including isoform 1 (168aa, 18kD), isoform 2 (125aa, 14kD). Rat CDKN2A has only one isoform (159aa, 17kD).

psi-iconProperties
PURIFICATION:CDKN2A Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CDKN2A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CDKN2A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CDKN2A
ALTERNATE NAMES:CDKN2A Antibody: ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P19ARF, P16INK4, P16INK4A, P16-INK4A, Cyclin-dependent kinase 4 inhibitor A
ACCESSION NO.:NP_000068
PROTEIN GI NO.:4502749
GENE ID:1029
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CDKN2A Antibody: The CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. Conversely, transfection of CDKN2A suppressed the growth of two independent mesothelioma cell lines, suggesting that inactivation of the CDKN2 gene is an essential step in the etiology of malignant mesotheliomas. CDKN2A induces a G1 cell cycle arrest by inhibiting the phosphorylation of the Rb protein by the cyclin-dependent kinases CDK4 and CDK6. CDKN2A is expressed as at least three distinct isoforms.
REFERENCES:1) Stone et al. Cancer Res. 1995; 55:2988-94.
2) Kamb et al. Nature Genet. 1994; 8:22-6.
3) Kratzke et al. J. Nat. Cancer Inst. 1995; 87:1870-5.
4) Stott et al. EMBO J. 1998; 17:5001-14.

ANTIBODIES FOR RESEARCH USE ONLY.

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