Datasheet

CDKN1C Antibody
CATALOG NUMBER: 7015

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (88%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:CDKN1C antibody can be used for detection of CDKN1C by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
PREDICTED MOLECULAR WEIGHT:35 kDa
SPECIFICITY:At least three isoforms of CDKN1C are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, CDKN1C often migrates at 57kDa in SDS-PAGE. CDKN1C antibody is predicted to not cross react with other CIP/KIP proteins.
IMMUNOGEN:CDKN1C antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CDKN1C.

The immunogen is located within amino acids 40 - 90 of CDKN1C.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CDKN1C Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CDKN1C Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CDKN1C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CDKN1C Antibody: BWS, WBS, p57, BWCR, KIP2, p57Kip2, Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57
ACCESSION NO.:NP_000067
PROTEIN GI NO.:4557441
OFFICIAL SYMBOL:CDKN1C
GENE ID:1028

Background

BACKGROUND:CDKN1C Antibody: The cyclin-dependent kinase inhibitor 1C (CDKN1C), also known as p57KIP2, is a tight-binding, strong inhibitor of several G1 cyclin/CDK complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndrome, a cancer-predisposing syndrome, suggesting that this gene is a tumor suppressor candidate. CDKN1C is widely expressed during organogenesis with its expression declining after maturity. It is part of the CIP/KIP family of cyclin-dependent inhibitors (CKIs) which also includes CDKN1A and CDKN1B.
REFERENCES: 1) Lee MH, Reynisdottir I, and Massague J. Cloning of p57KIP2, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 1995; 9:639-49.
2) Pateras IS, Apostolopoulou K, Niforou K, et al. p57KIP2: “Kip"ing the cell under control. Mol. Cancer Res. 2009; 1902-19
3) Hatada I, Ohashi H, Fukushima Y, et al. An imprinted p57KIP2 is mutated in Beckwith-Wiedemann syndrome. Nat. Genet. 1996; 14:171-3.

For Research Use Only