| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Mouse: (88%) |
| IMMUNOGEN: | CDKN1C antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CDKN1C. The immunogen is located within amino acids 40 - 90 of CDKN1C. |
| TESTED APPLICATIONS: | ELISA, IF, WB |
| APPLICATIONS: | CDKN1C antibody can be used for detection of CDKN1C by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | At least three isoforms of CDKN1C are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, CDKN1C often migrates at 57kDa in SDS-PAGE. CDKN1C antibody is predicted to not cross react with other CIP/KIP proteins. |
| POSITIVE CONTROL: | 1) Cat. No. 1210 - HEK293 Cell Lysate |
| 2) Cat. No. 17-010 - HEK293 Cell Slide | |
| PREDICTED MOLECULAR WEIGHT: | 35 kDa |
Specifications| PURIFICATION: | CDKN1C Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | CDKN1C Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | CDKN1C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | CDKN1C |
| ALTERNATE NAMES: | CDKN1C Antibody: BWS, WBS, p57, BWCR, KIP2, p57Kip2, Cyclin-dependent kinase inhibitor 1C, Cyclin-dependent kinase inhibitor p57 |
| ACCESSION NO.: | NP_000067 |
| PROTEIN GI NO.: | 4557441 |
| GENE ID: | 1028 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | CDKN1C Antibody: The cyclin-dependent kinase inhibitor 1C (CDKN1C), also known as p57KIP2, is a tight-binding, strong inhibitor of several G1 cyclin/CDK complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndrome, a cancer-predisposing syndrome, suggesting that this gene is a tumor suppressor candidate. CDKN1C is widely expressed during organogenesis with its expression declining after maturity. It is part of the CIP/KIP family of cyclin-dependent inhibitors (CKIs) which also includes CDKN1A and CDKN1B. |
| REFERENCES: | 1) Lee MH, Reynisdottir I, and Massague J. Cloning of p57KIP2, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 1995; 9:639-49. | 2) Pateras IS, Apostolopoulou K, Niforou K, et al. p57KIP2: “Kip"ing the cell under control. Mol. Cancer Res. 2009; 1902-19 | 3) Hatada I, Ohashi H, Fukushima Y, et al. An imprinted p57KIP2 is mutated in Beckwith-Wiedemann syndrome. Nat. Genet. 1996; 14:171-3. |
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