CDKN1B Antibody Cat. No.: 45-032

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human
HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow, Dog
IMMUNOGEN: The immunogen for this antibody is: C-EQTPKKPGLRRRQT
TESTED APPLICATIONS: ELISA, IP, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26-27kDa band observed in Human Breast and Prostate lysates (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14028-33; PMID: 16169901). Recommended concentration: 0.5-1.5ug/ml.Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
SPECIFICITY: #To_Delete#.
POSITIVE CONTROL:1) Cat. No. 1311 - Human Breast Tissue Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:CDKN1B
ALTERNATE NAMES:CDKN1B, P27KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1), KIP1, CDKN4, cyclin-dependent kinase inhibitor 1B, p27(KIP1), KIP1, p27, MEN1B, MEN4, P27KIP1
ACCESSION NO.:NP_004055.1
PROTEIN GI NO.:4757962
GENE ID:1027
psi-iconBackground and References
REFERENCES:1) Toyoshima H, Hunter T p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell. 1994 Jul 15;78(1):67-74.

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