Datasheet

CDCA8 Antibody
CATALOG NUMBER: 4751

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:CDCA8 antibody can be used for detection of CDCA8 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1465 - Rat Kidney Tissue Lysate
IMMUNOGEN:CDCA8 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of the human CDCA8.

The immunogen is located within the first 50 amino acids of CDCA8.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CDCA8 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CDCA8 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CDCA8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CDCA8 Antibody: BOR, DasraB, MESRGP, BOREALIN, PESCRG3, Borealin, Cell division cycle-associated protein 8, hDasra-B
ACCESSION NO.:NP_060571
PROTEIN GI NO.:8922438
OFFICIAL SYMBOL:CDCA8
GENE ID:55143

Background

BACKGROUND:CDCA8 Antibody: CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.
REFERENCES: 1) Gassmann R, Carvalho A, Hanzing AJ, et al. Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. J. Cell Biol.2004; 166:179-91.
2) Tani M, Okino N, Mitsutake S, et al. Molecular Cloning of the Full-length cDNA Encoding Mouse Neutral Ceramidase. J. Biol. Chem.2000; 275:11229-34.
3) Hanson KK, Kelley AC, and Bienz M Loss of Drosophila borealin causes polyploidy, delayed apoptosis and abnormal tissue development. Development2005; 132:4777-87.
4) Date DA, Jacob CJ, Bekier ME et al. Borealin is repressed in response to p53/Rb signaling. Cell Biol. Int.2007; 31:1470-81.

For Research Use Only