Datasheet

CDC42 Antibody
CATALOG NUMBER: 5101

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%), Bovine: (100%), Guinea pig: (100%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:CDC42 antibody can be used for detection of CDC42 by Western blot at 0.5 - 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
IMMUNOGEN:CDC42 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CDC42.

The immunogen is located within amino acids 40 - 90 of CDC42.
HOST SPECIES:Chicken

Properties

PURIFICATION:CDC42 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CDC42 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CDC42 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CDC42 Antibody: G25K, CDC42Hs, Cell division control protein 42 homolog, G25K GTP-binding protein
ACCESSION NO.:NP_001782
PROTEIN GI NO.:4757952
OFFICIAL SYMBOL:CDC42
GENE ID:998

Background

BACKGROUND:CDC42 Antibody: CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to S. cerevisiae CDC42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. CDC42 is thought to regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. At least two isoforms of CDC42 are known to exist.
REFERENCES: 1) Erickson JW and Cerione RA. Multiple roles for Cdc42 in cell regulation. Curr. Opin. Cell Biol.2001; 13:153-7.
2) Chen W, Lim HH, and Lim L. The CDC42 homologue from Caenorhabditis elegans. Complementation of yeast mutation. J. Biol. Chem.1993; 268:13280-5.
3) Hart MJ, Eva A, Evans T, et al. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. Nature1991; 354:311-4.
4) Rohatgi R, Ma L, Miki H, et al. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell1999; 97:221-31.

For Research Use Only