Cdc2 (phospho Tyr15) Antibody Cat. No.: XPS-1003

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Dog, Human, Mouse, Rat, Xenopus
IMMUNOGEN: Cdc2 (Tyr15) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Tyr15 of human, mouse, rat and xenopus cdc2.
TESTED APPLICATIONS: WB
APPLICATIONS: The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of SK-N-MC total cell extracts with phospho- and non-phosphopeptide competition were used to establish the phospho-specificity of the antibody. Immunolabeling of the Mr 34 kDa cdc2 protein is blocked by the Ser15 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) has not been determined. Drosophila, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
SPECIFICITY: Cdc2 antibody is specific for cdc2 phosphorylated at Tyr15.
PREDICTED MOLECULAR WEIGHT: 34

psi-iconProperties
PURIFICATION:Affinity Purified
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
STORAGE CONDITIONS:For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:Clk2
ALTERNATE NAMES:LOC365842, CDC-like kinase 2, Protein Clk2
ACCESSION NO.:Q5XI98
PROTEIN GI NO.:81910362
GENE ID:365842
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2.
REFERENCES:1) Sible, J.C., "Cell biology: thanks for the memory," Nature (London), 426 (2003) 392-393.
2) Sagata, N., "Molecular biology: untangling checkpoints," Science, 298 (2002) 1905-1907.
3) Nakamizo, A., Inamura, T., Inoha, S., Amano, T., Ochi, H., Ikezaki, K., and Fukui, M., "Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines," J. Neurooncol., 59 (2002) 7-13.
4) Maller, J.L., "Mitotic control," Curr. Opinion. Cell Biology, 3 (1991) 269-275.

ANTIBODIES FOR RESEARCH USE ONLY.

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