| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Dog, Human, Mouse, Rat, Xenopus |
| IMMUNOGEN: | Cdc2 (Tyr15) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Tyr15 of human, mouse, rat and xenopus cdc2. |
| TESTED APPLICATIONS: | WB |
| APPLICATIONS: | The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of SK-N-MC total cell extracts with phospho- and non-phosphopeptide competition were used to establish the phospho-specificity of the antibody. Immunolabeling of the Mr 34 kDa cdc2 protein is blocked by the Ser15 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) has not been determined. Drosophila, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB. |
| SPECIFICITY: | Cdc2 antibody is specific for cdc2 phosphorylated at Tyr15. |
| PREDICTED MOLECULAR WEIGHT: | 34 |
Specifications| PURIFICATION: | Affinity Purified |
| CLONALITY: | Polyclonal |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
| STORAGE CONDITIONS: | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| OFFICIAL SYMBOL: | Clk2 |
| ALTERNATE NAMES: | LOC365842, CDC-like kinase 2, Protein Clk2 |
| ACCESSION NO.: | Q5XI98 |
| PROTEIN GI NO.: | 81910362 |
| GENE ID: | 365842 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2. |
| REFERENCES: | 1) Sible, J.C., "Cell biology: thanks for the memory," Nature (London), 426 (2003) 392-393. | 2) Sagata, N., "Molecular biology: untangling checkpoints," Science, 298 (2002) 1905-1907. | 3) Nakamizo, A., Inamura, T., Inoha, S., Amano, T., Ochi, H., Ikezaki, K., and Fukui, M., "Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines," J. Neurooncol., 59 (2002) 7-13. | 4) Maller, J.L., "Mitotic control," Curr. Opinion. Cell Biology, 3 (1991) 269-275. |
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