| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Bovine: (95%) |
| IMMUNOGEN: | CD81 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81. The immunogen is located within amino acids 30 - 80 of CD81. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | CD81 antibody can be used for detection of CD81 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
| 2) Cat. No. 10-101 - Human Lung Tissue Slide | |
| 3) Cat. No. 17-003 - A549 Cell Slide | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 26 kDa Observed: 25 kDa |
Specifications| PURIFICATION: | CD81 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | CD81 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | CD81 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | CD81 |
| ALTERNATE NAMES: | CD81 Antibody: S5.7, CVID6, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, Tspan-28 |
| ACCESSION NO.: | NP_004347 |
| PROTEIN GI NO.: | 4757944 |
| GENE ID: | 975 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | CD81 Antibody: CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family, a group of cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that associates with integrins and appears to promote muscle cell fusion and support myotube maintenance. Along with SCARB1, LDL-R, and CLDN1, CD81 has been reported to be an entry factor for the Hepatitis C virus. Finally, recent evidence indicates that the CD81 gene is localized in a tumor-suppressor gene region and is thus a candidate gene for malignancies. |
| REFERENCES: | 1) Oren R, Takahashi S, Doss C, et al. TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol. Cell Biol. 1990; 10:4007-15. | 2) Charrin S, le Naour F, Silvie O, et al. Lateral organization of membrane proteins: tetraspanins spin their web. Biochem. J. 2009; 420:133-54. | 3) Mannion BA, Berditchevski F, Kraeft SK, et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). J. Immunol. 1996; 157:2039-47. | 4) Tacvhibana I and Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J. Cell Biol. 1999; 146:893-90. |
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