Datasheet

CD59 Antibody
CATALOG NUMBER: 7235

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (86%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:CD59 antibody can be used for detection of CD59 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1406 - Mouse Spleen Tissue Lysate
PREDICTED MOLECULAR WEIGHT:14 kDa
IMMUNOGEN:Rabbit polyclonal CD59 antibody was raised against a 14 amino acid peptide near the amino terminus of human CD59.

The immunogen is located within the first 50 amino acids of CD59.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CD59 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CD59 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CD59 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CD59 Antibody: 1F5, EJ16, EJ30, EL32, G344, MIN1, MIN2, MIN3, MIRL, HRF20, MACIF, MEM43, MIC11, MSK21, 16.3A5, HRF-20, MAC-IP, p18-20, CD59 glycoprotein, 1F5 antigen
ACCESSION NO.:NP_000602
PROTEIN GI NO.:10835165
OFFICIAL SYMBOL:CD59
GENE ID:966

Background

BACKGROUND:CD59 Antibody: The complement regulatory protein CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis and is involved in lymphocyte signal transduction. CD59 is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and ultimately cerebral infarction.
REFERENCES: 1) Venneker GT and Asghar SS. CD59: a molecule involved in antigen presentation as well as downregulation of membrane attack complex. Exp. Clin. Immunogenet. 1992; 9:33-47.
2) Kimberly FC, Sivasankar B, and Paul Morgan B. Alternative roles for CD59. Mol. Immunol. 2007; 44:73-81.
3) Ninomiya H and Sims PJ. The human complement regulatory protein CD59 binds to the alpha-chain of C8 and to the “b” domain of C9. J. Biol. Chem. 1992; 267:13675-80.
4) Deckert M, Ticchioni M, Mari B, et al. The glycosylphosphatidylinositol-anchored CD59 protein stimulates both T cell receptor zeta/ZAP-70-dependent and –independent signaling pathways in T cells. Eur. J. Immunol. 1995; 25:1815-22

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