CD47 Recombinant Protein Cat. No.: 96-849

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:HEK293 cells
SEQUENCE:Gln 19 - Pro 139
FUSION TAG:mouse IgG2a Fc tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha (HPLC-verified), His Tag at 2 ug/mL (100 uL/well) can bind Human CD47, mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.5-8 ng/mL (QC tested).
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.

Endotoxin level is less than 0.1 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:40.8 kDa
PHYSICAL STATE:Lyophilized
BUFFER:50 mM Tris, 100 mM Glycine, pH7.5
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:CD47,MER6,IAP,OA3
ACCESSION NO.:NP_942088
OFFICIAL SYMBOL:CD47
GENE ID:961
psi-iconBackground and References
BACKGROUND:Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
REFERENCES:1) Lindberg F.P., et al., 1993, J. Cell Biol. 123:485-496.
2) Latour S., et al., 2001, J. Immunol. 167:2547-2554.
3) Piccio L., et al., 2005, Blood 105:2421-2427.

FOR RESEARCH USE ONLY.

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