| HOST SPECIES: | Mouse |
| SPECIES REACTIVITY: | Chimpanzee, Human |
| IMMUNOGEN: | Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody. |
| TESTED APPLICATIONS: | Flow, IF, IHC |
| APPLICATIONS: | Flow Cytometry: 0.5-1 ug/million cells Immunofluorescence: 0.5-1 ug/ml Immunohistology (FFPE): 0.25-0.5 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance. 1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
Specifications| PURIFICATION: | Protein G affinity chromatography |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG2a, kappa |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| CONCENTRATION: | 0.2 mg/mL |
| STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
| OFFICIAL SYMBOL: | PTPRC |
| ALTERNATE NAMES: | Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45 |
| GENE ID: | 5788 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
| BACKGROUND: | Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. |
ANTIBODIES FOR RESEARCH USE ONLY.
For additional information, visit ProSci's Terms & Conditions Page.
