CD40L multimeric Recombinant Protein Cat. No.: 90-289

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psi-iconSpecifications
SPECIES:Human, Mouse, Rat
SOURCE SPECIES:CHO cells
SEQUENCE:Rat CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a DDDDK-tag.
FUSION TAG:DDDDK Tag
TESTED APPLICATIONS:
APPLICATIONS:This recombinant proteins is for research use only.
BIOLOGICAL ACTIVITY: Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86).
psi-iconProperties
PURITY:>95% (SDS-PAGE).

Endotoxin level is less than 0.02EU/ μg purified protein (LAL test; Lonza).
PHYSICAL STATE:Lyophilized
BUFFER:Contains PBS. Reconstitute with 100 μl sterile water.
CONCENTRATION:0.1mg/ml after reconstitution.
STORAGE CONDITIONS:Stable for at least 6 months after receipt when stored at -20˚C.
psi-iconAdditional Info
ALTERNATE NAMES:ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L
ACCESSION NO.:Q9Z2V2
PROTEIN GI NO.:21362974
OFFICIAL SYMBOL:CD40 ligand
GENE ID:84349
psi-iconBackground and References
BACKGROUND:MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has been shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.

FOR RESEARCH USE ONLY.

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