| SPECIES: | Human, Mouse |
| SOURCE SPECIES: | CHO cells |
| SEQUENCE: | Mouse CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a DDDDK-tag. |
| FUSION TAG: | DDDDK Tag |
| TESTED APPLICATIONS: | |
| APPLICATIONS: | This recombinant proteins is for research use only. |
| BIOLOGICAL ACTIVITY: | Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86). |
Specifications| PURITY: | >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | Contains PBS. Reconstitute with 100 μl sterile water. |
| CONCENTRATION: | 0.1mg/ml after reconstitution. |
| STORAGE CONDITIONS: | Stable for at least 6 months after receipt when stored at -20˚C. |
| ALTERNATE NAMES: | ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L |
| ACCESSION NO.: | P27548 |
| OFFICIAL SYMBOL: | Cd40lg |
| GENE ID: | 21947 |
| BACKGROUND: | MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced. |
FOR RESEARCH USE ONLY.
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