CD40 Ligand Recombinant Protein Cat. No.: 96-984

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psi-iconSpecifications
SPECIES:Mouse
SOURCE SPECIES:HEK293 cells
SEQUENCE:Gly 115 - Leu 260
FUSION TAG:His Tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Mouse CD40, Fc Tag at 2 ug/mL (100 uL/well) can bind Mouse CD40 Ligand, His Tag with a linear range of 5-20 ng/mL (QC tested).
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:16.8 kDa
PHYSICAL STATE:Lyophilized
BUFFER:PBS, pH7.4
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
ACCESSION NO.:P27548
OFFICIAL SYMBOL:CD40 Ligand
GENE ID:21947
psi-iconBackground and References
BACKGROUND:CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, alpha5beta1 integrin and alphaIIbbeta3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
REFERENCES:1) Schönbeck U, Libby P, 2001, Cell. Mol. Life Sci. 58 (1): 4–43.
2) Furman M.I. et al., 2004, J. Am. Coll. Cardiol. 43: 2319-25.
3) Spriggs, M.K. et al., 1992, J. Exp. Med. 176:1543.
4) Fanslow, W.C. et al., 1994, Seminars in Immunology 6:267. 

FOR RESEARCH USE ONLY.

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