CD4 Antibody [9H5A8] Cat. No.: PM-5201

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: WB: 0.5-4 μg/mL; IHC: 5 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1314 - Human Thymus Tissue Lysate
 2) Cat. No. 1205 - Jurkat Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 51kD

Observed: 51-57 kD (Post-modyfication: 2-4 N-linked glycosylations)
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 1): Anti-CD4 antibodies (PM-5201) detected human CD-4 recombinant protein at 5ng.

ISOFORMS: Human CD4 has one isoform (458aa, 51kD). Mouse CD4 has two isoforms, including isoform 1 (457aa, 51kD) and isoform 2 (217aa, 24.5kD). Rat CD4 has one isoform (457aa, 51kD). PM-5201 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:CD4 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CD4 monoclonal antibody can be stored at -20˚C, stable for one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:CD4
ALTERNATE NAMES:CD4 Antibody [9H5A8] : CD4mut
ACCESSION NO.:NP_000607
PROTEIN GI NO.:10835167
GENE ID:920
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CD4 Monoclonal Antibody: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.
REFERENCES:1) Bowers et al. Int. J. Biochem. Cell Biol. 1997; 29 :871-5.
2) Arthos et al. Cell 1989; 57:469-81.
psi-iconCITATIONS
CITATIONS: 1)Hood et al. Cytolytic nanoparticles attenuate HIV-1 infectivity. Antivir Ther. 2013;18(1):95-103. PMID: 22954649

ANTIBODIES FOR RESEARCH USE ONLY.

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