CD3e Antibody Cat. No.: 33-082

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Bovine, Cat, Chicken, Dog, Frog, Guinea Pig, Hamster, Horse, Human, Mouse, Opossum, Pig, Primate, Rabbit, Rat, Sheep
IMMUNOGEN: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
TESTED APPLICATIONS: Flow, IP, WB
APPLICATIONS: Flow Cytometry: use the CD3e antibody at 0.5-1 ug/million cells

WB: 0.5-1.0 ug/ml

IP: 1-2ug/500ug protein lysate



The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.

psi-iconProperties
PURIFICATION:Protein A purified CD3e antibody
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:CD3E
ALTERNATE NAMES:T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
GENE ID:916
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.

ANTIBODIES FOR RESEARCH USE ONLY.

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