CD33 Antibody Cat. No.: 6595

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: CD33 antibody was raised against a 14 amino acid synthetic peptide near the center of human CD33.

The immunogen is located within amino acids 180 - 230 of CD33.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
SPECIFICITY: At least three isoforms of CD33 are known to exist; this antibody will detect all three isoforms.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate

psi-iconProperties
PURIFICATION:CD33 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CD33 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CD33 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CD33
ALTERNATE NAMES:CD33 Antibody: p67, SIGLEC3, SIGLEC-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3
ACCESSION NO.:NP_001763
PROTEIN GI NO.:130979981
GENE ID:945
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CD33 Antibody: CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family that is highly expressed on myeloid progenitor cells. Assessment of CD33 expression is of great importance in the immunodiagnosis of acute leukemia, allowing distinction between myeloid and lymphoid origin, as CD33 is generally restricted to the myelomonocytic lineage. CD33 can associate with the protein-tyrosine phosphatases SHP-1 and SHP-2 and thus could modulate downstream signaling events associated with cell activation. Common variants of CD33 have been found to be associated with late-onset Alzheimer's disease.
REFERENCES:1) Freeman SD, Kelm S, Barber EK, et al. Characterization of CD33 as a new member of the sialoadhesion family of cellular interaction molecules. Blood 1995; 85:2005-12.
2) Crocker PR and Varki A. Siglecs, sialic acids, and innate immunity. Trends Immunol. 2001; 22:337-42.
3) Taylor VC, Buckley CD, Douglass M, et al. The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2. J. Biol. Chem. 1999; 274:11505-12
4) Naj AC, Jun G, Beecham GW, et al. Common variants at MS4A4/MS4A6E, CD2AP, CD33 and EPHA are associated with late-onset Alzheimer’s disease. Nat. Genet. 2011; 43:436-41

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