Datasheet

CD28 Antibody [37.51]
CATALOG NUMBER: 76-741

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Mouse
TESTED APPLICATIONS:Flow, Func, IP
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
SPECIFICITY:The 37.51 monoclonal antibody specifically reacts with mouse CD28 molecule, a 45 kDa homodimer.
HOST SPECIES:Hamster

Properties

PURIFICATION:The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
PHYSICAL STATE:liquid
BUFFER:Phosphate-buffered aqueous solution, ph7.2.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:The product should be stored undiluted at 4˚C . Do not freeze.
CLONALITY:Monoclonal
ISOTYPE:Golden Syrian Hamster IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CH29-189K17.3, Cd28
OFFICIAL SYMBOL:Cd28
GENE ID:12487

Background

BACKGROUND:The 37.51 monoclonal antibody specifically reacts with mouse CD28 molecule, a 45 kDa homodimer. It is expressed on most thymocytes, at low density on most of the CD4+ and CD8+ peripheral T lymphocytes, and on the natural killer cells. It is additionally found on activated splenocytes, mast cells, and thymocytes. CD 28 is a costimulator of T lymphocytes and a ligand for CD80 (B7-1), and CD86 (B7-2). The CD28 signaling stimulates the expression of IL-2 and IL-2 receptor and the cytotoxicity of CD3-activated T lymphocytes.The 37.51 antibody stimulates the proliferation of T lymphocytes and natural killer cells and their respective cytokine production.
REFERENCES: 1) Tacke, M., Clark, G. J., Dallman, M. J., Hnig, T. (1995). Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ester.The Journal of Immunology,154(10), 5121-5127.
2) Tacke, M., Hanke, G., Hanke, T., Hnig, T. (1997). CD28‐mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: Evidence for functionally distinct forms of CD28.European journal of immunology,27(1), 239-247.
3) Bluestone, J. A. (1995). New perspectives of CD28-B7-mediated T cell costimulation.Immunity,2(6), 555-559.

For Research Use Only