Datasheet

CD276 Antibody
CATALOG NUMBER: 8689

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CD276 antibody can be used for Western blot at 0.5 - 1 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 1406 - Mouse Spleen Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 35, 43, 57 kDa

Observed: 59 kDa
SPECIFICITY:At least three antibodies are known to exist; this antibody will detect all three isoforms.
IMMUNOGEN:CD276 antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of CD276.

The immunogen is located within amino acids 484 - 534 of CD276.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CD276 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CD276 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CD276 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CD276 Antibody: CD276 molecule, B7H3, B7-H3, B7RP-2, 4Ig-B7-H3
ACCESSION NO.:NP_001019907
PROTEIN GI NO.:67188443
OFFICIAL SYMBOL:CD276
GENE ID:80381

Background

BACKGROUND:CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
REFERENCES: 1) Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2001; 2:269-74.
2) Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes. J Immunol 2002; 168:6294-7.
3) Yi KH and Chen L. Fine tuning the immune response through B7-H3 and B7-H4. Immunol Rev 2009; 229:145-51.
4) Wang L, Kang FB, and Shan BE. B7-H3-mediated tumor immunology: friend or foe?. Int J Cancer 2014; 134:2764-71.

For Research Use Only