The extracellular domain of human CD275 [B7-H2] (aa 1-256) is fused to the N-terminus of the Fc region of mouse IgG2a.
FUSION TAG:
Fc Tag
TESTED APPLICATIONS:
APPLICATIONS:
This recombinant proteins is for research use only.
BIOLOGICAL ACTIVITY:
Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3.
Properties
PURITY:
>98% (SDS-PAGE).
Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza).
PHYSICAL STATE:
Lyophilized
BUFFER:
Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μl sterile water. Add 1X PBS to the desired protein concentration.
STORAGE CONDITIONS:
Stable for at least 1 year after receipt when stored at -20˚C. Working aliquots are stable for up to 3 months when stored at -20˚C.
Additional Info
ALTERNATE NAMES:
B7-H2, ICOS Ligand, B7-like Protein Gl50, B7RP-1
ACCESSION NO.:
NP_056074
PROTEIN GI NO.:
27477039
OFFICIAL SYMBOL:
ICOSLG
GENE ID:
23308
Background and References
BACKGROUND:
Human B7-H2 is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Specifications
