| SPECIES: | Mouse |
| SOURCE SPECIES: | CHO cells |
| SEQUENCE: | The extracellular domain of mouse CD275 [B7-H2] (aa 48-279) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a. |
| FUSION TAG: | Fc Tag |
| TESTED APPLICATIONS: | |
| APPLICATIONS: | This recombinant proteins is for research use only. |
| BIOLOGICAL ACTIVITY: | Measured by its binding ability in a functional ELISA. Immobilized human ICOS at 1ug/ml (100ul/well) can bind biotinylated B7-H2 (mouse):Fc (mouse) (rec.) (non-lytic) with a linear range of 0.1-1.0ug/ml. Optimal dilutions should be determined by each laboratory for each application. |
Specifications| PURITY: | ≥98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza). |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | Lyophilized from 0.2um-filtered solution in PBS. Reconstitute at 100 μg/ml in sterile PBS. |
| STORAGE CONDITIONS: | Stable for at least 1 year after receipt when stored at -20˚C. Working aliquots are stable for up to 3 months when stored at -20˚C. |
| ALTERNATE NAMES: | B7-H2, ICOS Ligand, B7-like Protein Gl50, B7RP-1 |
| ACCESSION NO.: | NP_056605 |
| PROTEIN GI NO.: | 7657220 |
| OFFICIAL SYMBOL: | Icosl |
| GENE ID: | 50723 |
| BACKGROUND: | Mouse CD275 (B7-H2) is a member of the growing B7 family of immune costimulatory proteins. CD275 has been identified as the ligand for ICOS, a member of the CD28 family of co-stimulatory receptors. Mouse CD275 is expressed on resting B cells and at low levels on monocytes. The CD275/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation. |
FOR RESEARCH USE ONLY.
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