CD271 Antibody [CDLA271] Cat. No.: 33-970

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: Recombinant human protein was used as the immunogen for the CD271 antibody.
TESTED APPLICATIONS: Flow, IF, IHC-P
APPLICATIONS: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the CD271 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

SPECIFICITY: Does not react with Mouse or Rat

psi-iconProperties
PURIFICATION:Protein G affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG1, kappa
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:NGFR
ALTERNATE NAMES:NGFR Antibody: CD271, p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, Tumor necrosis factor receptor superfamily member 16, NGF receptor
GENE ID:4804
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:CD271/NGFR pays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]

ANTIBODIES FOR RESEARCH USE ONLY.

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