CD152 [CTLA-4] non-lytic Recombinant Protein Cat. No.: 90-414

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:CHO cells
SEQUENCE:The extracellular domain of human CD152 [CTLA-4] (aa 37-160) is fused to the N-terminus of the Fc region of a mutant human IgG1.
FUSION TAG:Fc Tag
TESTED APPLICATIONS:
APPLICATIONS:This recombinant proteins is for research use only.
BIOLOGICAL ACTIVITY: Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells.
psi-iconProperties
PURITY:≥98% (SDS-PAGE).

Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza).
PHYSICAL STATE:Lyophilized
BUFFER:Lyophilized from 0.2um-filtered solution in PBS. Reconstitute at 100 μg/ml in sterile PBS.
STORAGE CONDITIONS:Stable for at least 1 year after receipt when stored at -20˚C. Working aliquots are stable for up to 3 months when stored at -20˚C.
psi-iconAdditional Info
ALTERNATE NAMES:CTLA-4, Cytotoxic T-lymphocyte Protein 4
ACCESSION NO.:NP_005205
PROTEIN GI NO.:21361212
OFFICIAL SYMBOL:CTLA4
GENE ID:1493
psi-iconBackground and References
BACKGROUND:CD152 (CTLA-4) and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD152 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD152 and CD28 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD152 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. CD152 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CD152 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CD152 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CD152 and CD28 can bind to the same ligands, CD152 binds to B71 and B72 with 20-100-fold higher affinity than CD28.

FOR RESEARCH USE ONLY.

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