CD11b Antibody [M1/70] (APC-Cy7) Cat. No.: 76-136

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psi-iconSpecifications
HOST SPECIES:Rat
SPECIES REACTIVITY: Human, Mouse
TESTED APPLICATIONS: Flow
SPECIFICITY: The M1/70 monoclonal antibody specifically reacts with the 170 kDa alphaM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18).

psi-iconProperties
PURIFICATION:The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
CLONALITY:Monoclonal
ISOTYPE:Rat IgG2b, kappa
CONJUGATE:APC-Cy7
PHYSICAL STATE:liquid
BUFFER:Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:The product should be stored undiluted at 4˚C and should be protected from prolonged exposure to light. Do not freeze.

psi-iconAdditional Info
OFFICIAL SYMBOL:ITGAM
ALTERNATE NAMES:CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, ITGAM
GENE ID:3684
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The M1/70 monoclonal antibody specifically reacts with the 170 kDa alphaM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18). Mac-1 binds to C3bi, CD54 (ICAM-1), and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.
REFERENCES:1) Sanchez-Madrid, F., Simon, P., Thompson, S., Springer, T. A. (1983). Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.The Journal of experimental medicine,158(2), 586-602.
2) Ault, K. A., Springer, T. A. (1981). Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells.The Journal of Immunology,126(1), 359-364.
3) Springer, T., Galfre, G., Secher, D. S., Milstein, C. (1978). Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.European journal of immunology,8(8), 539-551.

ANTIBODIES FOR RESEARCH USE ONLY.

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