CD117 Recombinant Protein Cat. No.: 96-839

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:HEK293 cells
SEQUENCE:Gln 26 - Thr 516
FUSION TAG:Fc Tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human CD117, Fc Tag at 2 ug/mL (100 uL/well) can bind Mouse SCF, His Tag with a linear range of 0.05-1.2 ng/mL (Routinely tested).
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:81.9 kDa
PHYSICAL STATE:Lyophilized
BUFFER:Tris with Glycine, Arginine and NaCl, pH7.5
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:CD117,SCFR,c-Kit,KIT
ACCESSION NO.:P10721-2
OFFICIAL SYMBOL:CD117
GENE ID:3815
psi-iconBackground and References
BACKGROUND:Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.
REFERENCES:1) Neumann I., et al., 2010, Pediatr. Blood Cancer 55:464-470.
2) Blume-Jensen P., et al., 1994, J. Biol. Chem. 269:21793-21802.
3) Sun J., et al.,2009, J. Biol. Chem. 284:11039-11047.

FOR RESEARCH USE ONLY.

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