CCP110 Antibody Cat. No.: 6825

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: CCP110 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human CCP110.

The immunogen is located within the last 50 amino acids of CCP110.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: CCP110 antibody can be used for detection of CCP110 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
SPECIFICITY: Two isoforms of CCP110 are known to exist; this antibody will only detect the longer isoform
POSITIVE CONTROL:1) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 110 kDa

Observed: 110 kDa

psi-iconProperties
PURIFICATION:CCP110 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:CCP110 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CCP110 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CCP110
ALTERNATE NAMES:CCP110 Antibody: CP110, Cep110, CEP110, CP110, KIAA0419, Centriolar coiled-coil protein of 110 kDa, Centrosomal protein of 110 kDa
ACCESSION NO.:NP_001185951
PROTEIN GI NO.:312222737
GENE ID:9738
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CCP110 Antibody: CCP110 is a centrosomal protein required for the centrosome to function as a microtubule organizing center and plays an essential role in centrosome duplication. It is a target of cyclin-dependent kinases (CDKs); disruption of CCP110 phosphorylation leads to unscheduled centrosome separation and polyploidy. CCP110 is also a target of SCF (Cyclin F) ubiquitin ligase complex, leading to its degradation, suggesting that this complex controls centrosome homeostasis and mitotic fidelity through CCP110 degradation. The centriolar kinesin Kif24 also interacts with CP110 and remodel microtubules and regulate ciliogenesis.
REFERENCES:1) Chen Z, Indjeian VB, McManus M, et al. CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells. Dev. Cell 2002; 3:339-50.
2) D’Angiolella V, Donato V, Vijayakumar S, et al. SCF (Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation. Nature 2010; 466:138-42.
3) Kobayashi T, Tsang WY, Li J, et al. Centriolar kinesin Kif24 interacts with CP110 to remodel microtubules and regulate ciliogenesis. Cell 2011; 145:914-25.

ANTIBODIES FOR RESEARCH USE ONLY.

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