Datasheet

CCNT1 Antibody
CATALOG NUMBER: 7223

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CCNT1 antibody can be used for detection of CCNT1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 80 kDa
IMMUNOGEN:Rabbit polyclonal CCNT1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human CCNT1.

The immunogen is located within the last 50 amino acids of CCNT1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CCNT1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CCNT1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CCNT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CCNT1 Antibody: CCNT, CYCT1, HIVE1, Cyclin-T1, CycT1
ACCESSION NO.:NP_001231
PROTEIN GI NO.:17978466
OFFICIAL SYMBOL:CCNT1
GENE ID:904

Background

BACKGROUND:CCNT1 Antibody: Cyclins function as regulators of CDK kinases and exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin-T1 protein (CCNT1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNT1 tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing CCNT1 and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. CCNT1 and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.
REFERENCES: 1) Uhlmann F, Bouchoux C, and Lopez-Aviles S. A quantitative model for cyclin-dependent kinase control of the cell cycle: revisited. Philos. Trans. R. Soc. Lond. B. biol. Sci. 2011; 366:3572-83.
2) Wei P, Garber ME, Fang SM, et al. A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell 1998; 92:451-62.
3) Mancebo HS, Lee G, Flygare J, et al. P-TEFb kinase is require for HIV Tat transcriptional activation in vivo and in vitro. Genes Dev. 1997 ; 11 :2633-44.
4) Majello B and Napolitano G. Control of RNA polymerase II activity by dedicated CTD kinases and phosphatases. Front. Biosci. 2001; 6:D1358-68.

For Research Use Only