Datasheet

CCDC98 Antibody
CATALOG NUMBER: 4321

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (93%), Chicken: (87%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:CCDC98 antibody can be used for detection of CCDC98 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1311 - Human Breast Tissue Lysate
 2) Cat. No. 10-001 - Human Breast Tissue Slide
IMMUNOGEN:CCDC98 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human CCDC98.

The immunogen is located within amino acids 80 - 130 of CCDC98.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CCDC98 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CCDC98 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CCDC98 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CCDC98 Antibody: ABRA1, CCDC98, ABRA1, UNQ496/PRO1013, BRCA1-A complex subunit Abraxas, Coiled-coil domain-containing protein 98
ACCESSION NO.:NP_620775
PROTEIN GI NO.:109148531
OFFICIAL SYMBOL:FAM175A
GENE ID:84142

Background

BACKGROUND:CCDC98 Antibody: CCDC98, also known as Abraxas 1, was identified through protein binding studies using the breast and ovarian predisposition protein BRCA1 as the binding target. CCDC98 recruits RAP80, a ubiquitin-binding protein, to BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both CCDC98 and RAP80 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either CCDC98 or RAP80 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that CCDC98 and RAP80 control only part of the DNA damage response role of BRCA1. At least two isoforms of CCDC98 are known to exist.
REFERENCES: 1) Wang B, Matsuoka S, Balliff BA, et al. Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science2007; 316:1194-1198.
2) Kim H, Huang J, and Chen J. CCDC98 is a BRCA1-BRCT domain-binding protein involved in the DNA damage response. Nat. Struct. Mol. Biol.2007; 14:710-5.
3) Liu Z, Wu J, and Yu X. CCDC98 targets BRCA1 to DNA damage sites. Nat. Struct. Mol. Biol.2007; 14:716-20.

For Research Use Only