Datasheet

CCDC134 Antibody
CATALOG NUMBER: 5265

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CCDC134 antibody can be used for detection of CCDC134 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:CCDC134 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CCDC134.

The immunogen is located within the first 50 amino acids of CCDC134.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CCDC134 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CCDC134 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CCDC134 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CCDC134 Antibody: dJ821D11.3, Coiled-coil domain-containing protein 134
ACCESSION NO.:NP_079097
PROTEIN GI NO.:13376216
OFFICIAL SYMBOL:CCDC134
GENE ID:79879

Background

BACKGROUND:CCDC134 Antibody: The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.
REFERENCES: 1) Steinmetz MO, Jelesarov I, Matousek WM, et al. Molecular basis of coiled-coil formation. Proc. Natl. Acad. Sci. USA2007; 104:7062-7.
2) Huang J, Shi T, Ma T, et al. CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK. Cell. Mol. Life Sci.2008; 65:338-49.

For Research Use Only