Datasheet

CBX4 Antibody
CATALOG NUMBER: 7007

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:CBX4 antibody can be used for detection of CBX4 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:62 kDa
SPECIFICITY:Two alternatively spliced transcript variants have been observed.
IMMUNOGEN:CBX4 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CBX4.

The immunogen is located within amino acids 50 - 100 of CBX4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CBX4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CBX4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CBX4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CBX4 Antibody: PC2, NBP16, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Pc2
ACCESSION NO.:NP_003646
PROTEIN GI NO.:55770830
OFFICIAL SYMBOL:CBX4
GENE ID:8535

Background

BACKGROUND:CBX4 Antibody: Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1). CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.
REFERENCES: 1) Simon JA and Tamkun JW. Programming off and on states in chromatin: mechanisms of Polycomb and trithorax group complexes. Curr. Opin. Genet. Dev. 2002;12:210-8.
2) Gieni RS and Hendzel MJ. Polycomb group protein gene silencing, non-coding RNA, stem cells, and cancer. Biochem. Cell Biol. 2009; 87:711-46.
3) Luis NM, Morey L, Mejetta S, et al. Regulation of human epidermal stem cell proliferation and senescence requires polycomb-dependent and -independent functions of Cbx4. Cell. Stem Cell 2011; 9:233-46.
4) Ismail IH, Gagné JP, Caron MC, et al. CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA damage. Nucleic Acids Res. 2012 Mar 8.

For Research Use Only